The Multi-Faceted Therapeutic Efficacy of Pomegranate Peel Extract (PPE) In Zebrafish (Danio Rerio) Models: An Integrated Analysis of Phytochemical Profiles, Antimicrobial Resistance, And Neurobehavioral Dynamics
Keywords:
Pomegranate Peel Extract, Zebrafish, Phytochemicals, NeurobehaviorAbstract
The quest for novel phytochemical interventions in the management of infectious diseases and neurological disorders has led to an increased interest in agricultural byproducts. Among these, Pomegranate Peel Extract (PPE), derived from Punica granatum, stands out due to its dense concentration of bioactive polyphenols. This study investigates the therapeutic potential of PPE using the zebrafish (Danio rerio) as a primary in vivo model. Zebrafish provide a unique advantage for high-throughput screening due to their genetic homology to humans and transparent embryonic stages. Our research integrates three distinct pillars: a comprehensive phytochemical characterization, an assessment of antimicrobial efficacy against common aquatic and human pathogens (including Mycobacterium marinum and Vibrio species), and an evaluation of neurobehavioral impacts following PPE exposure. Results indicate that PPE possesses significant antioxidant and antimicrobial properties that mitigate the progression of granulomatous infections. Furthermore, neurobehavioral assays, including the novel tank test and light-dark preference, suggest that PPE exerts a stabilizing effect on anxiety-like behaviors without inducing motor toxicity. This integrated assessment underscores PPE as a viable candidate for developing natural therapeutic agents for both aquaculture health and translational human medicine.
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